A study was conducted with a view to demonstrate the bacterial loads and identification of the bacterial organisms contaminating fast food items in the retail outlets of Sylhet metropolitan area and in around Sylhet Agricultural University, Sylhet. Bacteriological investigation was conducted on 64 samples of 4 different types of fast food. The result was expressed in cfug-1 of sample. Mean value of Total Viable Count (TVC) per gram of fast food items Vegetable singara, Shamucha, Potato chop and Vegetable roll were found to be 104.5×109, 93.5×109, 100.5×109 and 67×109 respectively collected from Baluchor Bazar, Uposhohor, Zindabazar and Shibgonj. The highest mean value of TVC was 104.5× 109 from Vegetable singara and the lowest mean value of TVC was 67 ×109 from Vegetable roll. The antibiotic sensitivity was done in this study and found that isolated Escherichia coli was highly sensitive to Chloramphenical, Gentamycin and Ceftriaxone, moderately sensitive to Gentamycin and Ceftriaxone and resistant to Amoxicillin, Ceftriaxone Nalidixic Acid and Bacteria.