The study was conducted at the IPM Laboratory of Entomology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh during July to December 2013, to evaluate the critical storage time of fresh eggs and parasitized eggs of Sitotroga cerealella Olivier (Pyralidae: Lepidoptera) by three Trichogramma spp. on the emergence of Trichogramma chilonis Ishii (Trichogrammatidae: Hymenoptera), Trichogramma evanescens West and Trichogramma japonicum Ashmead. It was observed in the study that the adult emergence of three species was higher in stored parasitized host eggs than that of stored fresh host eggs. The stored fresh host eggs showed more than 90% adult emergence up to 20 days from stored parasitized host eggs of T. evanescens in all the cases. Stored parasitized host eggs gave 50% adult emergence up to 60 days in case of three species. In contrast, the stored fresh host eggs showed more than 50% adult emergence only up to 30 days in all the cases. The adult emergence of the three Trichogramma species reduced gradually with the increase of storage period of parasitized eggs and fresh host eggs.