A study was conducted to evaluate the performance of broccoli genotypes under inorganic and organic culture system during October 2013 to March 2014. Three broccoli genotypes were used for the experiment. The study with inorganic was conducted at the Horticulture Research Field of Sylhet Agricultural University (SAU), Sylhet while the same experiment under organic culture system was conducted at the field of FIVDB (Friends in Village Development Bangladesh), Surmagate, Sylhet. Thus, the two systems were considered as two factors totalling 6 combinations with three replications. The data were statistically analyzed following Randomized Complete Block Design (RCBD). The whole experiment area (separately for inorganic and organic) was divided into three blocks, representing three replications. Each block consisted of three plots. Thus, total numbers of plots were nine for each culture system. Different morphological characteristics, yield and yield attributes were largely influenced by the genotypes. The highest individual curd weight (183.87 g plant-1) and secondary curd weight (136.05 g plant-1) were recorded from Premium while both of these were lowest for BR001. The mean weight of individual curd (138.01 g plant-1) and secondary curd weight (144.81 g plant-1) of broccoli in the inorganic culture system were significantly higher than under the organic production system. This difference could be attributed due to higher supply of nutrient in the inorganic culture system than organic culture system. Again the genotype Premium performed better in respect of individual curd weight (265.67 g plant-1) and secondary curd weight (216.36 g plant-1) in inorganic culture system while both were lowest (39.29 and 33.81 g plant-1, respectively) for the genotype BR001 when grown in organic culture system. Therefore, the genotype Premium can be recommended as a genotype for cultivation under both organic and inorganic culture systems.