This study was conducted to evaluate the water quality parameters, total bacterial load, and proximate analysis of biofloc using different feeding levels in the biofloc rearing system of Nile tilapia (Oreochromis niloticus). The experiment was set up fiberglass tank filled with 100 liters of fresh water in four treatments (T1, T2, T3, and T4) with two replications of each treatment. The tested treatments were namely T1 (control), T2 (normal dose of feed+floc), T3 (20%feed reduction+floc), and T4 (30% feed reduction+floc). The vital water quality parameters like temperature, pH, dissolved oxygen, ammonia, nitrite, nitrate, TDS, TSS, and alkalinity were monitored at 3-day intervals. The results for temperature and pH did not show any significant differences among all the treatments. The highest DO, ammonia, nitrite, and nitrate were observed in the control treatment (7.36, 1.78, 0.13, and 11.0 mg/L, respectively). The results for nitrite and nitrate showed highly significant differences among all the treatments. The total bacterial load was estimated, and Bacillus spp was found in all biofloc treatments. Proximal analysis showed differences in crude protein, crude lipids, and ash content among all the treatments, where the highest protein percentage was 41.03%, and the highest crude lipid and ash values were 12.21% and 16%, respectively. The results indicate that Biofloc technology is a sustainable way of enhancing water quality in aquaculture through microbial biomass production, which results in higher production of tilapia.