Cellulases are the enzymes which hydrolyse cellulosic biomass and are being produced by the microorganisms grown over cellulosic substrates. The cellulase producing fungal isolates were obtained from sea sands and cellulosic waste materials. Four fungal species were isolated and screened by using Carboxy Methyl Cellulose (CMC) agar medium as a selective medium. Production of clear zones by the fungal isolates on CMC agar medium supplemented with 1% CMC was considered as indicative of extracellular cellulase activity. The size of transparent zone diameter was considered as proportional to the level of cellulase production. These fungal isolates were identified as Aspergillus sp., Penicillium sp. and Fusarium sp. by studying their microscopic and macroscopic characters. A basal medium containing CMC, KH2PO4, K2HPO4, MgSO4, (NH4)2SO4, CaCl2 and FeSO4 at pH 7.0 was used for cellulase production. The assay of cellulase in term of CMCase was performed by measuring the release of reducing sugar. The crude cellulase produced by these fungal isolates was partially characterized. Optimum temperature for maximum cellulytic activity was 35°C and was active at 35°-40°C. The cellulase activity was significantly active over a broad pH range from 5.0 to 8.0 having maximum activity at pH 6.0.