The experiment was carried out during November 2013 to May 2014 in commercial flower garden of Society and Human Development Organization Tangail (SAHDOT), Village-Shota Gobra, Upazilla-Mirzapur, and District-Tangail to investigate the vegetative and reproductive characteristics of different genotypes of gladiolus flower. The experiment included five gladiolus genotypes. The characteristics in the study included plant height, length and breadth of leaf, number of leaves plant-1, length of flowers, breadth of flower, weight of flower, weight of single stick, length of spike, length of rachis, flowers plant-1, days to reach 50% spike initiation, capsule and seed. The results indicated the existence of wide variability among the genotypes on their vegetative and reproductive characteristics along with yield and yield attributes. The plant height of white genotype was the highest (60.25 cm) and the orange genotype was the lowest (45.00 cm), respectively due to genotypes. The length of leaf was recorded the highest (41.75 cm) in white genotype and while the shortest length (34.32 cm) was recorded for red one. The breadths of leaves were highest (3.04 cm) in yellow and the lowest (1.55 cm) in orange genotype. The average number of leaves was the highest (12.30) in white genotype which is followed by red, violet, orange and yellow, respectively. Genotypes varied from 59.61 to 92.00 cm and 25.60 to 47.20 cm in spike length and rachis length, respectively. Number of flower was the highest (14.30) in white and the lowest (8.42) in orange genotype. Considering crossing parameters, variations were observed for length of capsule, breadth of capsule, number of seeds capsule-1 and weight of thousand seeds. Data indicated that all the crosses produced more or less gladiolus true seed. The number of seeds capsule-1 ranged from 15.10 to 38.45. The highest number of seeds capsule-1 (38.45) was produced by the cross Yellow × Red while the lowest number of seeds capsule-1 (15.10) was produced by the cross Violet × Red. The results clearly indicated that the white genotype has the best planting materials which may be planted for luxuriant growth of plants and production of excellent flowers and for production of true seed the cross Yellow × Red is the best cross.