This experiment was set up to define the critical situation of pregnant too early lactating cows concerned with hypocalcemia induced by rapid loss of Ca from blood to milk and negative energy balance. Three nutri-tonic mixtures comprised of eggshell, oyster shell, molasses, and ghee residue were well mixed and autoclaved before feeding lactating cows at the farmer’s level. The minerals were quantified by atomic absorption spectroscopy and flame photometry according to the EDTA titration and QPG methods. The concentration of Ca and P were found to be 5:1, 1.5:1, and 3.5:1 in nutri-tonic I, II and III supplements, in which nutri-tonic-I possessed 115% and 62% higher Ca than nutri-tonic-II and III. Conversely, nutri-tonic-II exhibited elevated P, Zn, Mg, and Ca concentrations compared to both nutri-tonic-I and III. Meanwhile, nutri-tonic-III demonstrated higher levels of Fe. Nutri-tonic I supplementation influenced the cows by causing a 22% higher DM intake (8.12 kg d^-1cow^-1). Unlike the CP intake, 84% higher ME was consumed by nutri-tonic-III fed cows than others. Nutri-tonic supplementation nourished and boosted the cows’ higher (p<0.001) milk production (16 – 30% Ld^-1) with more fat content (23-68%). The emitted equation for Ca (y = -1.5047x + 115.72; R² = 0.006) and P (y = 0.6028x + 81.238; R² = 0.008) accounted 116g Ca and 81g P in ration for 12-15 L milch cow. In particular, mineral deficiency leads to the suppressed immunity of early lactating cows. To recap, energy and mineral supplementation might mobilize Ca for the functioning of parathyroid and enhance innate immunity in late pregnant to early lactating cows to prevent milk fever.