The present study was conducted to evaluate the Artificial Insemination (AI) programof UpazillaLivestock Office and BRAC AI Enterprise at Sadullapur, Gaibandha, Bangladesh. The study was conducted at Sadullapurupazilla ofGaibandha district fromJuly, 2017 through June, 2018. The data were collected from the record books of Upazila Livestock Office and BRAC AI Enterprise, Sadullapur, Gaibandha. The performance of AI program was evaluated on the basis of conception rate and calving rate. Descriptive statistics were performed to estimate the conception rate and calving rate.Chi-square test was performed to check the difference of conception rate and calving rate between Upazilla Livestock office and BRAC AI Enterprise where confidence interval was 95%. The conception rate of AI program at Upazila Livestock Office was significantly higher than the BRAC AI Enterprise. The calving rate of AI program at Upazila Livestock Office andBRAC AI Enterprise were not significantly different. The Holstein Friesian (HF) is commonly used for AI program by upazilla livestock office and BRAC AI Enterprise followed by Sahiwal, Jersy and other crossbred. It was shown that the conception rate was significantly higher at Upazila Livestock Office than the BRAC AI Enterprise at Sadullapurupazilla in Gaibandha district.