CHARACTERIZATION OF CITRUS BACTERIAL SPOT BACTERIA THROUGH BIOCHEMICAL APPROACHES AND ITS CONTROL MEASURES

D Afrin, M F Hossain, S M Z Hasan, M Khalekuzzaman and B Sikdar
Journal of the Sylhet Agricultural University, Volume 3, Issue 2, 2016, Pages 315-323

Abstract:

Citrus species are among the most important fruit crops in the world. Citrus bacterial spot disease is caused by the bacteria Xanthomona axonopodis pv. citrumelo which may cause huge damage of citrus production. The pathogen was isolated and cultured on LB liquid media at pH 7.5 and bacterial growth developed within 48 to 72 hours. Colonies on the nutrient agar media were straw yellow and creamy in color. Isolated bacteria identified by Gram staining, Endospore staining, KOH and MacConey agar test and the result showed that isolated bacteria were gram negative. It also showed positive result in case of Catalase, Simmons citrate agar, TSI, Oxidase and KIA test. In case of carbohydrate test, highest OD (0.26) was observed in Maltose containing medium of the isolate. The isolate was tested against ten plant extracts and fifteen antibiotics. The isolate was highly sensitive against cefotaxime, neomycin, streptomycin, gentamicin, chloramphenicol, azithromycin antibiotics and the susceptibility zones were 28±0.5, 26±0.5, 23±0.5, 17±0.5, 20±0.5, 17±0.5 mm respectively. Antimicrobial activity of ten plant extracts showed that the isolateis susceptible to Momordica charantia extract, revealed a wide antibacterial spectrum against the bacterial strain.

Keywords:

Citrus bacterial spot, infectious disease, Xanthomonas axonopodis, gram negative bacteria, antimicrobial activity


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D Afrin, M F Hossain, S M Z Hasan, M Khalekuzzaman and B Sikdar. 2016. CHARACTERIZATION OF CITRUS BACTERIAL SPOT BACTERIA THROUGH BIOCHEMICAL APPROACHES AND ITS CONTROL MEASURES, Journal of the Sylhet Agricultural University 3(2): 315-323.