EFFECT OF DIFFERENT FEEDS ON IN VITRO METHANE EMISSION FROM CROSSBRED DAIRY COW

M N Hossain, M M Mia, M A Baset and M M H Khan
Journal of the Sylhet Agricultural University, Volume 3, Issue 2, 2016, Pages 159-172

Abstract:

This in vitro experiment was aimed to estimate the amount of methane emission and degradability of different types of roughage and concentrate feed from the crossbred dairy cows. Ten feed ingredients were used in this experiment; rice straw (Oryza sativa), napier grass (Pennisetum purpureum) and german grass (Echinochloa polystachya) were grouped as roughages and rice polish (Oryza sativa), wheat bran (Triticum aestivum) and broken rice (Oryza sativa) as energy rich concentrates and red lentil (Lens culinaris), grass pea (Lathyrus sativus), mung bean (Vigna radiata) and mustard oil cake (Brassica nigra) as protein rich concentrates according to their nutrient composition. Rumen fluids from 3 crossbred dairy cows (Local×Holstein Friesian) with average body weight of 300±20 kg were collected from them just after slaughtering. Buffered rumen fluid was incubated at 39±0.5°C temperature. A completely randomize design was followed to assess the in vitro degradability after 72 h incubation, gas production at 0, 3, 6, 12, 24, 36, 48, 72 h and methane emission at 24, 48 and 72 h of incubation. Significant differences were observed (P<0.001) among the in vitro dry matter degradability (IVDMD), in vitro organic matter degradability (IVOMD), gas and methane production. Among the roughages IVDMD and methane production was significantly higher (P<0.001) in german grass (72.08% and 110.56 ml g-1 DM) and lower in rich straw (42.66% and 12.45 ml g-1 DM) while among the energy rich concentrates they were significantly higher (P<0.001) in broken rice (83.72% and 76.33 ml g-1 DM) and lower in rich polish (38.87% and 23.56 ml g-1 DM). Beside this significantly higher (P<0.001) IVDMD and methane emission were observed in Grass peas (87.49% and 54.11 ml g-1 DM) and were lower mustard oil cake (67.89% and 49.78 ml g-1 DM) among the protein rich concentrates. Methane emission was negatively correlated with the level of crude protein and ether extract and was positively correlated with the level of nitrogen-free extract. It may be concluded that nitrogen-free extract plays important role in increasing methane emission from crossbred dairy cows whereas crude fiber and ether extract plays important role to abate.

Keywords:

in vitro, feed ingredients, methane emission, dairy cows


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M N Hossain, M M Mia, M A Baset and M M H Khan. 2016. EFFECT OF DIFFERENT FEEDS ON IN VITRO METHANE EMISSION FROM CROSSBRED DAIRY COW, Journal of the Sylhet Agricultural University 3(2): 159-172.