The research work was conducted to evaluate the effects of enzyme and protein supplementation on growth performance and hematological parameters in broiler chickens. A total of 80 (15 days old) broiler chicks were randomly divided into four groups (group A, B, C and D). Group A was considered as control, fed with commercial broiler ration, group B supplemented with protein (60% protein concentrate @ 10% with commercial ration), group C supplemented with an enzyme (Alquerzim (@ 1 g l-1 drinking water), and group D supplemented with both enzyme and protein with the same dose, respectively. The experiment was continued for the period of 21 days. Results revealed that body weights were increased significantly (p<0.05) in all groups and highest body weights were recorded in Group D. Growth rate was highest at the 1st week (15-21 days of age) of experiment than the 2nd week (22-28 days of age) and no significant increase was observed in the last week (29-35 day of age). No significant differences were observed among the groups for PCV and TEC values. Hemoglobin (g dl-1) content increased significantly (p<0.05) and ESR decreased significantly (p<0.05) in group D. Therefore, the study reveals that combined supplementation of protein and enzyme showed better result over control in respect to body weight gain, growth rate and hematological parameters. The research work was conducted to evaluate the effects of enzyme and protein supplementation on growth performance and hematological parameters in broiler chickens. A total of 80 (15 days old) broiler chicks were randomly divided into four groups (group A, B, C and D). Group A was considered as control, fed with commercial broiler ration, group B supplemented with protein (60% protein concentrate @ 10% with commercial ration), group C supplemented with an enzyme (Alquerzim (@ 1 g l-1 drinking water), and group D supplemented with both enzyme and protein with the same dose, respectively. The experiment was continued for the period of 21 days. Results revealed that body weights were increased significantly (p<0.05) in all groups and highest body weights were recorded in Group D. Growth rate was highest at the 1st week (15-21 days of age) of experiment than the 2nd week (22-28 days of age) and no significant increase was observed in the last week (29-35 day of age). No significant differences were observed among the groups for PCV and TEC values. Hemoglobin (g dl-1) content increased significantly (p<0.05) and ESR decreased significantly (p<0.05) in group D. Therefore, the study reveals that combined supplementation of protein and enzyme showed better result over control in respect to body weight gain, growth rate and hematological parameters.