Feeding experiment was conducted for 62-days with commercial probiotic “Navio plus” to identify and quantify its effects on growth and feed utilization parameters of Asian stinging catfish (Heteropneustes fossilis). Ten (10) H. fossilis fingerlings (3.63±0.02 g) were stocked in each aquarium and 12 aquaria were divided into four groups (3 aquaria/group). Fish groups were fed without probiotic, 2, 5 and 10g / kg probiotic which was marked as T1 (control), T2, T3 and T4 (treatments), respectively. At the end of the experiment, mean final body weight (FBW) of T1, T2, T3 and T4 was 12.92±0.01, 14.05±0.01, 15.67±0.01 and 16.21±0.01 g, respectively. Similarly, mean weight gain (WG) was 255.83±0.42, 287.05±0.27, 331.68±0.27 and 346.56±0.27%, respectively. Among these results, FBW and WG in all treatments were significantly (P < 0.05) higher than control (T1), where T4 had the highest value among the treatments. Like as FBW and WG, specific growth rate was also significantly increased in all treatment groups. In case of feed utilization parameters, feed conversion ratio and feed efficiency ratio among these diet groups remain unchanged (P > 0.05). However, protein efficiency ratio in T1 (1.30±0.100) was the lowest, whereas, T4 achieved the highest value as found in FBW and WG. Therefore, tested probiotic may be used at a dose higher than its company recommended dose to get higher growth as well as more production for profitable H. fossilis aquaculture in Bangladesh