Plant-growth-promoting rhizobacteria (PGPR) are beneficial microorganisms that dwell in the rhizospheric region and positively affect plants. In the present study, bacteria were isolated from the rhizosphere of healthy tomato plants, and their effects on plant growth and Fusarium oxysporum f. sp. Iycopersici were assayed. Sixteen isolates were obtained, among which five isolates, ERB4, ERBS, ERB12, ERB14, and ERB16, were capable of producing ammonia, IAA and phosphate solubilization. Meanwhile, they also showed in vitro antagonistic activities against F. oxysporum. The isolates were identified via morphology observation on selective and semi-selective media and 16S rRNA sequencing, ‘which were classified as Azotobacter sp., Bacillus sp., Pseudomonas stutzeri and Enterobacter cloacae. The present study demonstrates that these bacteria can act as PGPRs to stimulate plant growth and suppress plant pathogenic fungi. It is important to develop new biocontrol agents for Fusarium wilt of tomato.