The experiment was conducted at the Department of Biochemistry, Sher-E-Bangla Agricultural University, Dhaka and Bangladesh Agricultural Research Institute (BARI), Gazipur to investigate the chemical constituents of oils from rapeseed varieties Tori-7, SAU Sharisha-1 and Sampad, respectively. The highest (42.15%) oil content of seed was recorded in SAU Sharisha-1. On the other hand, the lowest (37.45%) oil content was recorded in the cultivar Sampad. The highest (185.13) saponification value was recorded in SAU Sharisha-1. On the other hand, the lowest (159.89) saponification value was recorded in Tori-7. The lowest (303.19) saponification equivalent was recorded in the SAU Sharisha-1, while the highest (350.88) saponification equivalent was recorded in Tori-7. The highest (0.55) acid value was recorded in Sampad, while the lowest (0.26) acid value was recorded in SAU Sharisha-1. The highest (110.00) iodine value was recorded in SAU Sharisha-1 and the lowest (104.50) iodine value was recorded in Sampad. The highest (0.75) unsaponifiable matter was recorded in Sampad, while the lowest (0.60) was recorded in Tori-7. The highest (4.38%) saturated fatty acid was recorded in SAU Sharisha-1, while the lowest (3.45%) was recorded in Tori-7. The highest (96.55%) total unsaturated fatty acid was recorded in Tori-7 and the lowest (90.43%) was recorded in SAU Sharisha-1.